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General Mastitis Information

A practical look at contagious Mastitis

National Mastitis Council Website

Canadian Bovine Mastitis Research Network Website

Mastitis 3 Factsheet


Mastitis PCR Assay - Finnzymes Diagnostics


NMC Annual Meeting Proceedings - January, 2010

Validation of PCR Mastitis assay for 3 major Contagious Pathogens

Field evaluation of Mastitis PCR assay with DHI sample


NMC Annual Meeting Proceedings - January, 2009

Comparison of real-time PCR and conventional bacterial culturing in bovine Mastitis testing

Real-time PCR-based identification of bacteria in milk samples from bovine clinical Mastitis with no growth in conventional culturing


Journal of Dairy Science - March, 2009

Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for indentifcation of bovine mastitis pathogens

Milk testing for contagious Mastitis is available to both DHI and non-DHI herds.Please contact your staff representative or call DHI at 1-800-549-4373
for more information.

The Mastitis 3 test identifies the presence of the three mastitis pathgens commonly referred to as the ‘contagious’ pathogens, specifically Staph. aureus, Strep. agalactiae and Mycoplasma bovis. These contagious pathogens are often the cause of chronic infections leading to ongoing elevated SCC.

Here’s some reasons why using the Mastitis 3 test makes good sense:

IT’S TARGETEDThe Mastitis 3 test identifies the presence of the three mastitis pathgens commonly referred to as the ‘contagious’ pathogens, specifically Staph. aureus, Strep. agalactiae and Mycoplasma bovis. These contagious pathogens are often the cause of chronic infections leading to ongoing elevated SCC. They can spread easily from cow to cow at milking time, and for Staph. aureus and Mycoplasma bovis, are difficult to treat.

IT’S RELIABLEThe test is based on polymerase chain reaction (PCR), which detects the presence of the bacteria’s DNA in the milk sample. You no longer have to rely on the ability of the bacteria to grow under culture conditions. The test has been validated with preserved metered samples, making it applicable to DHI services where mastitis
testing has not been possible before.

IT’S CONVENIENTThe regular DHI sample can be used! No more messing around with time consuming sample collection, storage and shipment.

IT’S FASTOnce samples are in the lab, results are usually available within a day.

IT’S FLEXIBLEYou have the ability to test the entire herd, selected cows (such as newly purchased or clinical cows), or cows that exceed a selected SCC level, where those samples will be redirected from the SCC analyzer to the Mastitis 3 test.

IT’S INTEGRATEDPositive test results, reported as +, ++, or +++ for each of the three pathogens, are displayed on an easy-to-read report and are integrated with other important SCC and DHI information for improved decision making.

For more information on the Mastitis 3 test, talk to your veterinarian, contact your local DHI field service representative or call DHI at 1-800-549-4373.

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